1W2L

Cytochrome c domain of caa3 oxygen oxidoreductase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Wavelength(s)	0.97, 1.7373
Spacegroup name	P 41 21 2
Unit cell lengths	54.049, 54.049, 91.347
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.300
R-factor	0.1423
R-free	0.16970
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	0.032
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.400
High resolution limit [Å]	1.300	1.300
Rmerge	0.060
Number of reflections	31867
<I/σ(I)>	14.73
Completeness [%]	98.4	90.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				1.5 M AMMONIUM SULPHATE, 0.2 M SODIUM ACETATE TRIHYDRATE