1W2I
Crystal structuore of acylphosphatase from Pyrococcus horikoshii complexed with formate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 85.336, 85.336, 75.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 73.920 - 1.500 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2acy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.199 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.950 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.390 |
| Number of reflections | 51268 | |
| <I/σ(I)> | 22.5 | 5.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.7 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 290 | 0.1M MES PH6 1.4M SODIUM FORMATE 17C, pH 6.00 |
