1W27
Phenylalanine ammonia-lyase (PAL) from Petroselinum crispum
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 100 |
| Collection date | 2003-11-15 |
| Wavelength(s) | 0.9798,0.9800,0.9824 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 119.960, 160.810, 141.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.19900 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.366 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.049 | 0.392 |
| Number of reflections | 156788 | |
| <I/σ(I)> | 21.4 | 4.5 |
| Completeness [%] | 96.8 | 88.2 |
| Redundancy | 7.1 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 8% PEG-6000, 40 MM MGCL2, pH 7.50 |
