1W26
Trigger Factor in Complex with the Ribosome forms a Molecular Cradle for Nascent Proteins
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Collection date | 2004-01-25 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.251, 47.415, 114.831 |
| Unit cell angles | 90.00, 113.66, 90.00 |
Refinement procedure
| Resolution | 45.910 - 2.700 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.32400 |
| Structure solution method | MAD |
| RMSD bond length | 0.001 |
| RMSD bond angle | 1.314 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.060 |
| Number of reflections | 26426 |
| <I/σ(I)> | 16 |
| Completeness [%] | 100.0 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 22-26% POLYETHYLENEGLYCOL 4000 WITH 14% 1,4-BUTANEDIOL IN 0.1M MES PH 7.0-7.5 |
