1W23
Crystal structure of phosphoserine aminotransferase from Bacillus alcalophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 144.467, 84.840, 67.469 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.080 |
| R-factor | 0.1168 |
| R-free | 0.13940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bjn |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.033 |
| Data reduction software | HKL |
| Data scaling software | HKL |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.110 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.050 | 0.370 |
| Number of reflections | 351228 | |
| <I/σ(I)> | 32.9 | 2.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 23.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 27.75% (V/V) PEG 400, 185 MM MAGNESIUM CHLORIDE HEXAHYDRATE, 7.5% (V/V) GLYCEROL AND 92.7 MM HEPES PH 7.5 |
