1W1S
Plant Cytokinin Dehydrogenase in Complex with Benzylaminopurine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Collection date | 2004-02-04 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 168.683, 168.683, 53.759 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.198 |
| Rwork | 0.197 |
| R-free | 0.21900 |
| Structure solution method | OTHER |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.998 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.130 | 0.400 |
| Number of reflections | 52429 | |
| <I/σ(I)> | 11.6 | 2.4 |
| Completeness [%] | 99.2 | 97.1 |
| Redundancy | 5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | pH 4.60 |
