1W0N
Structure of uncomplexed Carbohydrate Binding Domain CBM36
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.529, 40.187, 81.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.330 - 0.800 |
R-factor | 0.129 |
Rwork | 0.129 |
R-free | 0.14400 |
Structure solution method | OTHER |
RMSD bond length | 0.019 |
RMSD bond angle | 2.032 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 0.840 |
High resolution limit [Å] | 0.800 | 0.800 |
Rmerge | 0.060 | 0.370 |
Number of reflections | 95183 | |
<I/σ(I)> | 12 | 3 |
Completeness [%] | 97.0 | 97 |
Redundancy | 4 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | CRYSTALS OF NATIVE CBM36 (14.9 MG/ML) WERE GROWN USING THE VAPOR DIFFUSION TECHNIQUE FROM HANGING DROPS IN 1.6 M MAGNESIUM SULFATE AND 0.1 M MES, PH 6.5 |