1W0I

Arabidopsis thaliana Mitochondrial KAS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	200
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	72.218, 93.246, 74.007
Unit cell angles	90.00, 106.43, 90.00

Refinement procedure

Resolution	23.660 - 2.100
R-factor	0.178
Rwork	0.178
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ek4
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.600	2.190
High resolution limit [Å]	2.100	2.100
Rmerge	0.140	0.260
Number of reflections	54180
<I/σ(I)>	4.4	1.8
Completeness [%]	95.4	95.4
Redundancy	3.1	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		1.6 M (NH4)2SO4, 0.1 M MES-BUFFER PH 6.0