1W0I
Arabidopsis thaliana Mitochondrial KAS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.218, 93.246, 74.007 |
| Unit cell angles | 90.00, 106.43, 90.00 |
Refinement procedure
| Resolution | 23.660 - 2.100 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ek4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.600 | 2.190 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.140 | 0.260 |
| Number of reflections | 54180 | |
| <I/σ(I)> | 4.4 | 1.8 |
| Completeness [%] | 95.4 | 95.4 |
| Redundancy | 3.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 1.6 M (NH4)2SO4, 0.1 M MES-BUFFER PH 6.0 |
