1W0F
Crystal structure of human cytochrome P450 3A4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-10 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 77.410, 101.510, 128.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.000 - 2.650 |
| Rwork | 0.239 |
| R-free | 0.30320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w0e |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.080 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.045 | 0.328 |
| Number of reflections | 14804 | |
| <I/σ(I)> | 11.9 | 2.2 |
| Completeness [%] | 97.8 | 97.2 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 HEPES PH 7.5, 0.25M KCL, 12% PEG 4000, 5% MPD, 0.025M C |
