1W07

Arabidopsis thaliana acyl-CoA oxidase 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	200
Detector technology	CCD
Collection date	2003-02-02
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	85.201, 117.001, 131.047
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.590 - 2.000
R-factor	0.204
Rwork	0.204
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1IS2 DOMAIN 2 AND 3
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.500	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.110	0.490
Number of reflections	88554
<I/σ(I)>	7.4	2
Completeness [%]	95.8	95.2
Redundancy	2.3	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.2 M CALCIUM ACETATE, 0.1 M SODIUM COCADYLATE PH 6.5, 14% PEG 8000