1VZY
Crystal structure of the Bacillus subtilis HSP33
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Collection date | 2004-02-14 |
| Wavelength(s) | 1.28310,1.2837 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 115.292, 115.292, 106.441 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.970 |
| Rwork | 0.197 |
| R-free | 0.22500 |
| Structure solution method | MAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.848 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.050 | 0.512 |
| Number of reflections | 124827 | |
| <I/σ(I)> | 24.57 | 2.64 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.6 M K/NA TARTRATE, 0.1 M HEPES PH 7.5 |
