1VZY
Crystal structure of the Bacillus subtilis HSP33
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Collection date | 2004-02-14 |
Wavelength(s) | 1.28310,1.2837 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 115.292, 115.292, 106.441 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.970 |
Rwork | 0.197 |
R-free | 0.22500 |
Structure solution method | MAD |
RMSD bond length | 0.025 |
RMSD bond angle | 1.848 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.050 | 0.512 |
Number of reflections | 124827 | |
<I/σ(I)> | 24.57 | 2.64 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 1.6 M K/NA TARTRATE, 0.1 M HEPES PH 7.5 |