1VZW
Crystal structure of the bifunctional protein Pria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 64.473, 64.473, 102.869 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.800 |
| Rwork | 0.164 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h5y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.246 |
| Data reduction software | MOSFLM (V. 6.2.3) |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.300 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.083 | 0.510 |
| Number of reflections | 27751 | |
| <I/σ(I)> | 7.4463 | 1.41 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.93 | 2.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 1.6 M NH4SO4 PH 5.5 |
