1VYZ

Structure of CDK2 complexed with PNU-181227

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	53.250, 71.910, 72.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.430 - 2.210
Rwork	0.238
R-free	0.28000
Structure solution method	OTHER
RMSD bond length	0.013
RMSD bond angle	1.500
Data scaling software	SCALEPACK
Refinement software	CNX (2002)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.048	0.389
Number of reflections	14353
<I/σ(I)>	14	3.3
Completeness [%]	99.5	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4		10 PERCENT PEG4000, 50 MM AMMONIUN ACETATE, 50 MM HEPES PH 7.4