1VYU

Beta3 subunit of Voltage-gated Ca2+-channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Collection date	2003-11-15
Spacegroup name	P 1 21 1
Unit cell lengths	57.300, 66.400, 90.000
Unit cell angles	90.00, 103.10, 90.00

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.207
Rwork	0.207
R-free	0.24800
Structure solution method	OTHER
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.072	0.252
Number of reflections	27894
Completeness [%]	94.0	84
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.2		HEPES PH 7.2 250 MM NACL 15% POLYETHYLENE GLYCOL 3350