1VYT

beta3 subunit complexed with aid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Collection date	2004-03-15
Spacegroup name	C 1 2 1
Unit cell lengths	252.300, 69.000, 60.700
Unit cell angles	90.00, 96.70, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.231
Rwork	0.231
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vyu
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	GCOMO
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.065	0.319
Number of reflections	32147
Completeness [%]	96.0	77
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		TRIS-HCL PH 8.0, 150 MM MGCL2, 15 PEG3350