1VWA

STREPTAVIDIN-FSHPQNT

Experimental procedure

Temperature [K]	293
Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	I 2 2 2
Unit cell lengths	97.030, 107.470, 48.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.850
R-factor	0.192
Rwork	0.192
R-free	0.25900
RMSD bond length	0.017
RMSD bond angle	28.500 *
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.630 *
Rmerge	0.083
Total number of observations	98073 *
Number of reflections	25792
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4	20 *	Pahler, A., (1987) J. Biol. Chem., 262, 13933. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	30-32 (%sat)
2	1	reservoir	potassium acetate	0.1 (M)	pH4.0
3	1	drop	protein	15 (mg/ml)
4	1	drop	ammonium salfate	15-16 (%sat)
5	1	drop	potassium acetate	0.05 (M)