1VTJ
MOLECULAR STRUCTURE OF THE NETROPSIN-D(CGCGATATCGCG) COMPLEX: DNA CONFORMATION IN AN ALTERNATING AT SEGMENT; CONFORMATION 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 283 |
| Detector technology | DIFFRACTOMETER |
| Detector | NICOLET P3 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.480, 41.260, 66.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.202 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 1848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 283 | pH 6.50, VAPOR DIFFUSION, temperature 283.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | 2 | 3 |
| 2 | 1 | 1 | MPD | 5 | 6 |
| 3 | 1 | 1 | NA CACODYLATE | 8 | 9 |
| 4 | 1 | 1 | MGCL2 | 11 | 12 |
| 5 | 1 | 1 | SPERMINE | 14 | 15 |
| 6 | 1 | 2 | WATER | 17 | 18 |
| 7 | 1 | 2 | MPD | 20 | 21 |
