1VSV
Crystal Structure of holo-glyceraldehyde 3-phosphate dehydrogenase from Cryptosporidium parvum
Replaces: 3CIEExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2004-07-02 |
Detector | MAR300 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.119, 121.189, 80.326 |
Unit cell angles | 90.00, 92.21, 90.00 |
Refinement procedure
Resolution | 48.850 - 2.000 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vsu |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 87612 | |
<I/σ(I)> | 8.9 | |
Completeness [%] | 98.3 | 90.4 |
Redundancy | 3.1 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 298 | 20% PEG 3000, pH 7.5, vapor diffusion, hanging drop, temperature 298K, pH 7.50 |