1VSR
VERY SHORT PATCH REPAIR (VSR) ENDONUCLEASE FROM ESCHERICHIA COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-17 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.020, 54.240, 73.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.2 * |
| Rwork | 0.196 |
| R-free | 0.21600 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 2.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.030 | 0.177 |
| Number of reflections | 12869 | |
| <I/σ(I)> | 20.1 | |
| Completeness [%] | 94.8 | 91.4 |
| Redundancy | 2.45 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.5 | 20 * | CRYSTALLIZATION CONDITIONS: ROOM TEMPERATURE BATCH, 75 MM SODIUM PHOSPHATE PH 6.5, 150 MM NACL, 2 MM DTT, 10% PEG 4K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | sodium phosphate | 75 (mM) | |
| 2 | 1 | 1 | 150 (mM) | ||
| 3 | 1 | 1 | PEG4000 | 10 (%) | |
| 4 | 1 | 1 | protein | 10 (mg/ml) |
