1VSC

VCAM-1

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1994-04-08
Detector	SIEMENS
Spacegroup name	C 1 2 1
Unit cell lengths	122.090, 48.860, 73.440
Unit cell angles	90.00, 117.42, 90.00

Refinement procedure

Resolution	15.000 - 1.900
R-factor	0.204
Rwork	0.204
R-free	0.28900
RMSD bond length	0.016
RMSD bond angle	29.700 *
Data reduction software	BUDDHA
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.900
Rmerge	0.059
Number of reflections	25533
Completeness [%]	82.9
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.7 *		Wang, J., (1994) Proteins: Struct.,Funct., Genet., 20, 287. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	VCAM-D1D2	11 (mg/ml)
2	1	drop		12.5 (mM)
3	1	drop		125 (mM)
4	1	reservoir	PEG4000	20 (%(w/v))
5	1	reservoir	2-propanol	10 (%(v/v))
6	1	reservoir		0.1 (M)