1VS3

Crystal Structure of the tRNA Pseudouridine Synthase TruA From Thermus thermophilus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	298
Detector technology	CCD
Collection date	2005-07-12
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97924, 0.97954, 0.9700
Spacegroup name	P 41 21 2
Unit cell lengths	91.530, 91.530, 163.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.080 - 2.250
R-factor	0.222
Rwork	0.222
R-free	0.23400
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	2.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.250	2.250
Number of reflections	33977
Completeness [%]	100.0	99.9
Redundancy	15.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M Tris-HCl, 6% PEG2000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M Tris-HCl, 6% PEG2000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M Tris-HCl, 6% PEG2000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K