1VRU

HIGH RESOLUTION STRUCTURES OF HIV-1 RT FROM FOUR RT-INHIBITOR COMPLEXES

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	IMAGE PLATE
Collection date	1994-03
Detector	BAS-III
Spacegroup name	P 21 21 21
Unit cell lengths	143.200, 116.800, 66.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.400
R-factor	0.187
Rwork	0.187
RMSD bond length	0.016
RMSD bond angle	25.600 *
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.400	2.400 *
Rmerge	0.128
Total number of observations	218842 *
Number of reflections	38354	3050 *
Completeness [%]	86.5	60.7 *
Redundancy	5.71

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3400	6 (%)
2	1	reservoir	citrate/phosphate