1VRS
Crystal structure of the disulfide-linked complex between the N-terminal and C-terminal domain of the electron transfer catalyst DsbD
Replaces: 1SE1Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 98.2 |
| Detector technology | CCD |
| Collection date | 2003-08-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.751 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 188.460, 52.600, 107.920 |
| Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.850 |
| Rwork | 0.224 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jzd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.240 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 24336 | |
| <I/σ(I)> | 16.1 | 3.6 |
| Completeness [%] | 99.3 | 97.3 |
| Redundancy | 4.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | sodium acetate, sodium formate, PEG 2000 MME, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
