1VRD
Crystal structure of Inosine-5'-monophosphate dehydrogenase (TM1347) from THERMOTOGA MARITIMA at 2.18 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-21 |
| Detector | ADSC |
| Spacegroup name | I 4 |
| Unit cell lengths | 120.083, 120.083, 144.079 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.120 - 2.180 |
| R-factor | 0.21863 |
| Rwork | 0.217 |
| R-free | 0.25759 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zfj |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.523 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.120 | 2.200 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Number of reflections | 50043 | |
| <I/σ(I)> | 14.26 | 1.51 |
| Completeness [%] | 94.5 | 75.52 |
| Redundancy | 4.11 | 3.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 4 | 273 | 40% MPD, 0.1M citric acid pH 4.0, final pH 4, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 273K |
