1VRB

Crystal structure of Putative asparaginyl hydroxylase (2636534) from Bacillus subtilis at 2.60 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-09-22
Detector	ADSC
Spacegroup name	P 21 21 21
Unit cell lengths	47.300, 104.660, 286.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.660 - 2.600
R-factor	0.22399
Rwork	0.221
R-free	0.27227
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.595
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXD (+ AutoSHARP)
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.660	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.067	0.727
Number of reflections	49807
<I/σ(I)>	13.7	2.3
Completeness [%]	98.6	93.3
Redundancy	4.6	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP		293	24% NP_PEG 4000, 0.065M Tris_base, 0.16M Mg Cl, 0.035M Tris Cl, 20% Glycerol, CuCl2, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP, NANODROP		293	24% NP_PEG 4000, 0.065M Tris_base, 0.16M Mg Cl, 0.035M Tris Cl, 20% Glycerol, CuCl2, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP, NANODROP		293	24% NP_PEG 4000, 0.065M Tris_base, 0.16M Mg Cl, 0.035M Tris Cl, 20% Glycerol, CuCl2, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K