1VR0
Crystal structure of putative 2-phosphosulfolactate phosphatase (15026306) from Clostridium acetobutylicum at 2.6 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-19 |
Detector | ADSC |
Wavelength(s) | 1.020035,0.979834 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 46.686, 69.193, 453.518 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.700 - 2.490 |
R-factor | 0.19201 |
Rwork | 0.190 |
R-free | 0.22938 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.509 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.530 |
High resolution limit [Å] | 2.490 | 2.490 |
Number of reflections | 22838 | |
<I/σ(I)> | 24.38 | 3.19 |
Completeness [%] | 85.4 | 29.22 |
Redundancy | 4.5 | 1.34 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.5 | 277 | 20.0% PEG-8000, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.5 | 277 | 20.0% PEG-8000, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |