1VQS

Crystal structure of a nipsnap family protein with unknown function (atu4242) from agrobacterium tumefaciens str. c58 at 1.50 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-09
Detector	ADSC
Spacegroup name	I 4 2 2
Unit cell lengths	94.792, 94.792, 303.133
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.970 - 1.500
R-factor	0.1523
Rwork	0.151
R-free	0.17681
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.460
Data reduction software	MOSFLM
Data scaling software	SCALA (CCP4 4.2)
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.970	1.540
High resolution limit [Å]	1.500	1.500
Number of reflections	110198
<I/σ(I)>	13.2	1.7
Completeness [%]	100.0	100
Redundancy	6.3	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	14% PEG MME 2000, 0.175M Sulfate_NH4, 0.05M Tris_base, 0.05M Tris Cl, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	14% PEG MME 2000, 0.175M Sulfate_NH4, 0.05M Tris_base, 0.05M Tris Cl, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	14% PEG MME 2000, 0.175M Sulfate_NH4, 0.05M Tris_base, 0.05M Tris Cl, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K