1VQR

Crystal structure of a virulence factor (cj0248) from campylobacter jejuni subsp. jejuni at 2.25 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-11
Detector	ADSC
Spacegroup name	C 1 2 1
Unit cell lengths	123.141, 122.090, 96.897
Unit cell angles	90.00, 120.58, 90.00

Refinement procedure

Resolution	61.040 - 2.250
R-factor	0.19519
Rwork	0.193
R-free	0.23286
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.355
Data reduction software	MOSFLM
Data scaling software	SCALA (4.2))
Phasing software	SHELXD
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.050	2.310
High resolution limit [Å]	2.250	2.250
Number of reflections	56900
<I/σ(I)>	11.4	1.7
Completeness [%]	97.4	90.4
Redundancy	2.7	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	277	20.0% PEG-6000, 1.0M LiCl, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K