1VQE

GENE V PROTEIN MUTANT WITH VAL 35 REPLACED BY ILE 35 AND ILE 47 REPLACED BY MET 47 (V35I, I47M)

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-05
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	76.120, 28.150, 42.450
Unit cell angles	90.00, 103.47, 90.00

Refinement procedure

Resolution	5.000 - 1.800
R-factor	0.218
Rwork	0.218
RMSD bond length	0.013
RMSD bond angle	2.640
Data reduction software	MAR (600)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.043
Number of reflections	8006
Completeness [%]	97.3
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.6 *		Skinner, M.M., (1994) Proc.Nat.Acad.Sci.USA, 91, 2071. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	drop	Tris-HCl	0.025 (M)
3	1	drop	PEG4000	10 (%)
4	1	reservoir	PEG4000	10 (%)