1VPZ

Crystal structure of a putative carbon storage regulator protein (csra, pa0905) from pseudomonas aeruginosa at 2.05 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-10
Detector	ADSC
Spacegroup name	C 2 2 21
Unit cell lengths	49.066, 56.669, 98.533
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.590 - 2.050
R-factor	0.22092
Rwork	0.218
R-free	0.27545
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.568
Data reduction software	MOSFLM
Data scaling software	SCALA (4.2))
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.230	2.100
High resolution limit [Å]	2.050	2.050
Number of reflections	8735
<I/σ(I)>	11.4	1.7
Completeness [%]	97.8	85.4
Redundancy	3.5	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	3.7	277	30.0% MPD, 0.1M Phosphate Citrate pH 3.7, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	3.7	277	30.0% MPD, 0.1M Phosphate Citrate pH 3.7, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K