1VPK
Crystal structure of DNA polymerase III, beta subunit (TM0262) from Thermotoga maritima at 2.00 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-13 |
| Detector | ADSC |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 91.702, 91.702, 111.463 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.630 - 2.000 |
| R-factor | 0.19814 |
| Rwork | 0.196 |
| R-free | 0.23207 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqj |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.668 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 32639 | |
| <I/σ(I)> | 39.82 | 2.25 |
| Completeness [%] | 99.8 | 99.65 |
| Redundancy | 10.66 | 8.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 4 | 293 | 6.25% PEG-6000, 0.1M Citrate pH 4.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
