1VPH

CRYSTAL STRUCTURE OF a YbjQ-like protein of unknown function (SSO2532) FROM SULFOLOBUS SOLFATARICUS P2 AT 1.76 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-18
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	79.860, 92.180, 114.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.220 - 1.764
R-factor	0.19082
Rwork	0.189
R-free	0.22173
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vmf
RMSD bond length	0.017
RMSD bond angle	1.607
Data scaling software	SCALA (5.0))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.680	1.860
High resolution limit [Å]	1.764	1.764
Number of reflections	75897
<I/σ(I)>	15.6	3
Completeness [%]	90.9	63.6
Redundancy	3.9	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	277	10.0% iso-Propanol, 20.0% PEG-4000, 0.1M HEPES pH7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K