1VP2
CRYSTAL STRUCTURE OF A PUTATIVE XANTHOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN HOMOLOG (TM0159) FROM THERMOTOGA MARITIMA AT 1.78 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-03 |
| Detector | ADSC |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 142.851, 142.851, 45.082 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.650 - 1.780 |
| R-factor | 0.16777 |
| Rwork | 0.166 |
| R-free | 0.20473 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v7r |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.592 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.650 | 1.830 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 37599 | |
| <I/σ(I)> | 12.6 | 1.8 |
| Completeness [%] | 83.6 | 42.4 |
| Redundancy | 4.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 1.6M (NH4)2SO4, 0.1M NaCl, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
