1VMH
Crystal structure of an uncharacterized conserved protein yjbq/upf0047 family, ortholog yugu b.subtilis (ca_c0907) from clostridium acetobutylicum at 1.31 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-08 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 |
| Unit cell lengths | 79.406, 79.406, 50.688 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.130 - 1.310 |
| R-factor | 0.15871 |
| Rwork | 0.157 |
| R-free | 0.18503 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vmf |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.339 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC ((5.2.0005)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.130 | 1.380 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Number of reflections | 27579 | |
| <I/σ(I)> | 14.6 | 1.7 |
| Completeness [%] | 96.3 | 85.5 |
| Redundancy | 5.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 9 | 277 | 2.0% Dioxane, 10.0% PEG-20000, 0.1M Bicine, pH 9.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |
