1VMB
Crystal structure of 30S ribosomal protein S6 (TM0603) from Thermotoga maritima at 1.70 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-24 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.815, 60.815, 76.543 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.620 - 1.700 |
| R-factor | 0.18549 |
| Rwork | 0.183 |
| R-free | 0.23735 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lou |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.643 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC ((5.2.0001)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.620 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 15840 | |
| <I/σ(I)> | 16.2 | 2.1 |
| Completeness [%] | 96.2 | 73.9 |
| Redundancy | 5.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 293 | 20.0% PEG-3350, 0.1M NaAcetate, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
