1VMB

Crystal structure of 30S ribosomal protein S6 (TM0603) from Thermotoga maritima at 1.70 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-24
Detector	ADSC QUANTUM 315
Spacegroup name	P 41 21 2
Unit cell lengths	60.815, 60.815, 76.543
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.620 - 1.700
R-factor	0.18549
Rwork	0.183
R-free	0.23735
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lou
RMSD bond length	0.018
RMSD bond angle	1.643
Data reduction software	MOSFLM
Data scaling software	SCALA (4.2))
Phasing software	MOLREP
Refinement software	REFMAC ((5.2.0001))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.620	1.740
High resolution limit [Å]	1.700	1.700
Number of reflections	15840
<I/σ(I)>	16.2	2.1
Completeness [%]	96.2	73.9
Redundancy	5.4	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		293	20.0% PEG-3350, 0.1M NaAcetate, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K