1VM7
Crystal structure of Ribokinase (TM0960) from Thermotoga maritima at 2.15 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-11 |
| Detector | ADSC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.142, 45.265, 77.260 |
| Unit cell angles | 90.00, 93.30, 90.00 |
Refinement procedure
| Resolution | 60.400 - 2.150 |
| R-factor | 0.19189 |
| Rwork | 0.190 |
| R-free | 0.23539 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gqt |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.477 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.400 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 39375 | |
| <I/σ(I)> | 10 | 1.3 |
| Completeness [%] | 92.3 | 61.2 |
| Redundancy | 3.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 293 | 0.4M (NH4)2Tartrate, 0.4% PEG-3350, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
