1VLI
Crystal structure of Spore coat polysaccharide biosynthesis protein spsE (BSU37870) from Bacillus subtilis at 2.38 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-05-20 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 70.578, 70.578, 205.359 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.290 - 2.380 |
R-factor | 0.19271 |
Rwork | 0.190 |
R-free | 0.23799 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.508 |
Data reduction software | XDS |
Data scaling software | SCALA (4.2)) |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.290 | 2.510 |
High resolution limit [Å] | 2.380 | 2.380 |
Number of reflections | 24673 | |
<I/σ(I)> | 13.5 | 2.3 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 6.9 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 18% PEG MME 2000, 0.04M Tris_base, 0.06M Tris Cl , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K | |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 18% PEG MME 2000, 0.04M Tris_base, 0.06M Tris Cl , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K | |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 18% PEG MME 2000, 0.04M Tris_base, 0.06M Tris Cl , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |