1VLG
Crystal structure of Ferritin (TM1128) from Thermotoga maritima at 2.00 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-07 |
| Detector | ADSC |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 175.634, 175.634, 354.843 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.730 - 2.000 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eum |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.288 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.730 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 140898 | |
| <I/σ(I)> | 16.1 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.4 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 277 | 2.4M (NH4)2SO4, 0.1M MES pH 6.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K, pH 6.00 |
