1VL8
Crystal structure of Gluconate 5-dehydrogenase (TM0441) from Thermotoga maritima at 2.07 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-20 |
| Detector | APS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 123.504, 123.504, 122.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.670 - 2.070 |
| R-factor | 0.15148 |
| Rwork | 0.150 |
| R-free | 0.17955 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g6k |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.655 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Number of reflections | 61222 | |
| <I/σ(I)> | 16.01 | 1.06 |
| Completeness [%] | 89.4 | 72.83 |
| Redundancy | 6.89 | 2.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 4.2 | 277 | Acetate pH 4.2, 35% Glycerol, 0.4M NH4H2PO3 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
