1VL5
CRYSTAL STRUCTURE OF A PUTATIVE METHYLTRANSFERASE (BH2331) FROM BACILLUS HALODURANS C-125 AT 1.95 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979494,0.885567,0.979494 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.006, 87.475, 105.236 |
| Unit cell angles | 90.00, 100.57, 90.00 |
Refinement procedure
| Resolution | 49.830 - 1.950 |
| R-factor | 0.1789 |
| Rwork | 0.177 |
| R-free | 0.20882 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.513 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.830 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 90667 | |
| <I/σ(I)> | 11.8 | 1.6 |
| Completeness [%] | 93.2 | 62.9 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 293 | 12% PEG MME 2000, 0.04M Acetic Acid, 0.06M Citrate_Na3 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
