1VL2
Crystal structure of Argininosuccinate synthase (TM1780) from Thermotoga maritima at 1.65 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-20 |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.322, 114.906, 149.606 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.210 - 1.650 |
| R-factor | 0.17902 |
| Rwork | 0.177 |
| R-free | 0.20940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kh1 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.627 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 195858 | |
| <I/σ(I)> | 22.21 | 2.17 |
| Completeness [%] | 100.0 | 99.87 |
| Redundancy | 4.91 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 293 | 16% PEG 3350, 0.20M Mg Acetate, 0.10M Imidazole , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K |
