1VL1
Crystal structure of 6-phosphogluconolactonase (TM1154) from Thermotoga maritima at 1.70A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-02 |
| Detector | ADSC |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.733, 75.146, 148.443 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.110 - 1.550 |
| R-factor | 0.16717 |
| Rwork | 0.165 |
| R-free | 0.20395 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pbt |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.620 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 24729 | |
| <I/σ(I)> | 16.59 | 2.93 |
| Completeness [%] | 79.5 | 26.04 |
| Redundancy | 3.86 | 2.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 277 | 0.2M (NH4)2HCitrate, 20% PEG-3350 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
