1VKU
Crystal structure of Acyl carrier protein (TM0175) from Thermotoga maritima at 2.00 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.898407, 0.979608, 0.979462 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 45.420, 45.420, 74.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.020 - 2.000 |
| R-factor | 0.19954 |
| Rwork | 0.197 |
| R-free | 0.25276 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.373 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.020 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 6172 | |
| <I/σ(I)> | 10.8 | 2.6 |
| Completeness [%] | 96.8 | 83.9 |
| Redundancy | 5.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.7 | 277 | 0.2M MgAcetate, 20% PEG-3350, pH 7.7, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
