1VKN

Crystal structure of N-acetyl-gamma-glutamyl-phosphate reductase (TM1782) from Thermotoga maritima at 1.80 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-09-20
Detector	ADSC
Spacegroup name	P 1 21 1
Unit cell lengths	85.098, 92.103, 100.880
Unit cell angles	90.00, 112.76, 90.00

Refinement procedure

Resolution	46.510 - 1.800
R-factor	0.17942
Rwork	0.178
R-free	0.21182
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.515
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0001)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.055	0.507
Number of reflections	131126
<I/σ(I)>	18.91	2.12
Completeness [%]	99.5	95.79
Redundancy	3.96	2.85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		295	13.50% PEG 3350, 0.50M (NH4)formate , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 295K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		295	13.50% PEG 3350, 0.50M (NH4)formate , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 295K