1VK8
CRYSTAL STRUCTURE OF A PUTATIVE THIAMINE BIOSYNTHESIS/SALVAGE PROTEIN (TM0486) FROM THERMOTOGA MARITIMA AT 1.80 A RESOLUTION
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-13 |
Detector | ADSC |
Wavelength(s) | 1.0332, 0.9796 |
Spacegroup name | P 1 |
Unit cell lengths | 47.646, 47.650, 49.608 |
Unit cell angles | 73.80, 62.90, 73.62 |
Refinement procedure
Resolution | 41.710 - 1.800 |
R-factor | 0.14404 |
Rwork | 0.142 |
R-free | 0.18956 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.443 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (4.2)) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.710 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 25897 | |
<I/σ(I)> | 10.2 | 3.7 |
Completeness [%] | 76.1 | 23.9 |
Redundancy | 1.9 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 9.6 | 277 | 20% PEG-8000, 0.1M CHES pH 9.5, pH 9.6, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K. cryo condition: 12% PEG 200. |