1VK1

Conserved hypothetical protein from Pyrococcus furiosus Pfu-392566-001

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-19
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	C 1 2 1
Unit cell lengths	115.148, 40.099, 65.671
Unit cell angles	90.00, 122.29, 90.00

Refinement procedure

Resolution	55.900 - 1.200
R-factor	0.166
Rwork	0.165
R-free	0.17500
Structure solution method	SINGLE WAVELENGTH ANOMALOUS SCATTERING
RMSD bond length	0.014
RMSD bond angle	1.298
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.03)
Refinement software	REFMAC (refmac_5.2.0001)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.240
High resolution limit [Å]	1.200	1.200
<I/σ(I)>	34.3	4.1
Completeness [%]	80.5	21.5
Redundancy	2.4	1.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	291	2.0M NaCl, 0.1M TRIS, pH 8.5, modified microbatch, temperature 291K