1VJX

Crystal structure of a putative ferritin-like diiron-carboxylate protein (tm1526) from thermotoga maritima at 2.30 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-20
Detector	APS-1
Wavelength(s)	0.979292, 0.964063, 0.979127
Spacegroup name	I 41 2 2
Unit cell lengths	82.333, 82.333, 121.641
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.060 - 2.300
R-factor	0.21979
Rwork	0.217
R-free	0.25652
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.469
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	9150
<I/σ(I)>	22.69	3.45
Completeness [%]	94.8	95.43
Redundancy	8.82	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	4.2	277	Phosphate Citrate pH 4.2, 10% 2-propanol, 0.2M Li2SO4 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K