1VJV

Crystal structure of Ubiquitin carboxyl-terminal hydrolase 6 (yfr010w) from Saccharomyces cerevisiae at 1.74 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-25
Detector	ADSC
Spacegroup name	P 21 21 21
Unit cell lengths	49.231, 79.449, 106.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.650 - 1.740
R-factor	0.18069
Rwork	0.179
R-free	0.20892
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.304
Data reduction software	MOSFLM
Data scaling software	SCALA (4.2))
Phasing software	SHELXD
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.450	1.790
High resolution limit [Å]	1.740	1.740
Rmerge	0.052	0.594
Number of reflections	42823
<I/σ(I)>	15.3	2.1
Completeness [%]	98.2	89.8
Redundancy	4	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	16% PEG MME 2000, 0.03M Tris_base, 0.07M Tris Cl , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	16% PEG MME 2000, 0.03M Tris_base, 0.07M Tris Cl , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP		277	16% PEG MME 2000, 0.03M Tris_base, 0.07M Tris Cl , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K