1VJU

Coproporphyrinogen III oxidase from Leishmania major

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Detector technology	CCD
Collection date	2004-02-08
Detector	ADSC QUANTUM 210
Spacegroup name	P 1
Unit cell lengths	52.649, 53.734, 66.627
Unit cell angles	86.46, 77.13, 61.50

Refinement procedure

Resolution	40.000 - 1.401
Rwork	0.151
R-free	0.18040
RMSD bond length	0.021
RMSD bond angle	2.522
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.06)
Refinement software	REFMAC (refmac_5.2.0000 24/04/2001)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.550	1.430
High resolution limit [Å]	1.401	1.400
Rmerge	0.074	0.510
Number of reflections	116623
<I/σ(I)>	7.64	2.1
Completeness [%]	95.1	92.9
Redundancy	3.85	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	0.4 ul protein 19 mg/ml, 0.4 ul crystallization buffer: 29% PEG 5000MME, 200 mM Ammonium sulfate, 100 mM MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K