1VJG
Crystal structure of a gdsl-like lipase (alr1529) from nostoc sp. pcc 7120 at 2.01 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-13 |
| Detector | ADSC |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 56.192, 56.192, 129.318 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.320 - 2.010 |
| R-factor | 0.17454 |
| Rwork | 0.172 |
| R-free | 0.21832 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.435 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.320 | 2.120 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Number of reflections | 15039 | |
| <I/σ(I)> | 13 | 3.4 |
| Completeness [%] | 90.6 | 65.7 |
| Redundancy | 5.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7.5 | 277 | 10% iso-propanol, 20% PEG-4000, 0.1M Sodium HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
